TY  - BOOK
AU  - Gong,Edwin Yunhao
TI  - Antiviral: methods and protocols 
T2  - Methods in Molecular Biology
SN  - 9781627034838 (alk. paper)
AV  - RM411 .A5755 2013
U1  - 615.7924 23
PY  - 2013///
CY  - New York
PB  - Humana Press, Springer
KW  - Antiviral Agents
N1  - Includes bibliographical references and index; Part I; Human immunodeficiency virus type 1 (HIV-1) --; Fluorescence-based high-throughput screening assay to identify HIV-1 inhibitors; Peggy Geluykens ... [et al.] --; Homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion; Liesbet Smeulders ... [et al.] --; Identification of HIV-1 reverse transcriptase inhibitors using a scintillation proximity assay; Bertrand Van Schoubroeck ... [et al.] --; Biochemical screening assays to identify HIV-1 integrase inhibitors; Marleen Clynhens ... [et al.] --; HIV-1 genotyping of the protease-reverse transcriptase and integrase genes to detect mutations that confer antiretroviral resistance; Peter Van den Eede ... [et al.] --; Part II; Hepatitis viruses --; In vitro kinetic profiling of hepatitis C virus NS3 protease inhibitors by progress curve analysis; Rumin Zhang and William T. Windsor --; Novel hepatitis C virus NS5B polymerase assay of de novo initiated RNA synthesis directed from a heteropolymeric RNA template; Eric Ferrari and Hsueh-Cheng Huang --; Selecting and characterizing drug-resistant hepatitis C virus replicon; Inge Vliegen, Leen Delang, and Johan Neyts --; Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors; Leen Vijgen ... [et al.] --; Expression and purification of hepatitis C virus protease from clinical samples; Liesbet van der Helm --; Surface plasmon resonance as a tool to select potent drug candidates for hepatitis C virus NS5B polymerase; Erna Cleiren, Benoit Devogelaere, and Katleen Fierens --; Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b; Diana Koletzki ... [et al.] --; Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures; Dawei Cai ... [et al.] --; In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates; Yang Liu and Kathryn M. Kitrinos --; Part III; Flaviviruses --; Cell-based antiviral assays for screening and profiling inhibitors against dengue virus; Edwin Yunhao Gong ... [et al.] --; Duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes; Edwin Yunhao Gong ... [et al.] --; High-throughput screening using dengue virus reporter genomes; Wolfgang Fischl and Ralf Bartenschlager --; Fluorimetric and HPLC-based dengue virus protease assays using a FRET substrate; Christoph Nitsche and Christian D. Klein --; Expression and purification of dengue virus NS5 polymerase and development of a high-throughput enzymatic assay for screening inhibitors of dengue polymerase; Edwin Yunhao Gong ... [et al.] --; Detection and quantification of flavivirus NS5 methyl- transferase activities; Siew Pheng Lim, Christophe Bodenreider, and Pei-Yong Shi --; Testing antiviral compounds in a dengue mouse model; Wouter Schul, Andy Yip, and Pei-Yong Shi --; Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds; Sofia L. Alcaraz-Estrada, Erin Donohue Reichert,  and Radhakrishnan Padmanabhan --; Part IV; Herpes viruses --; Methods for screening and profiling inhibitors of herpes simplex viruses; Edwin Yunhao Gong --; In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models; Frances Valencia, Ronald L. Veselenak, and Nigel Bourne --; Fluorescence-based high-throughput screening assay for identifying human cytomegalovirus inhibitors; Christel Van den Eynde ... [et al.] --; Part V; Respiratory viruses --; Fluorescence-based high-throughput antiviral compound screening assay against respiratory syncytial virus; Leen Kwanten, Ben De Clerck, and Dirk Roymans --; Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells; Anna Lundin, Tomas Bergström, and Edward Trybala --; Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models; Joke Van den Berg, Leen Kwanten, and Dirk Roymans --; In vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (Chlorocebus pygerythrus) infection model; Lieselot Houspie ... [et al.] --; Methods to determine mechanism of action of anti-influenza inhibitors; Angela Luttick, Stephanie Hamilton, and Simon P. Tucker --; Methods for evaluation of antiviral efficacy against influenza virus infections in animal models; Donald F. Smee and Dale L. Barnard --; Part VI; Alphaviruses --; Development of a high-throughput antiviral assay for screening inhibitors of chikungunya virus and generation of drug-resistant mutations in cultured cell; Edwin Yunhao Gong ... [et al.] --; Mouse model of chikungunya virus with utility in antiviral studies; Ashley Dagley and Justin G. Julander
ER  -