methods and protocols Gong, Edwin Yunhao. text bibliography nyu New York Humana Press Springer c2013 2013 2nd ed. monographic eng

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xiv, 452 p. : ill. (some col.) ; 26 cm. Part I Human immunodeficiency virus type 1 (HIV-1) -- Fluorescence-based high-throughput screening assay to identify HIV-1 inhibitors / Peggy Geluykens ... [et al.] -- Homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion / Liesbet Smeulders ... [et al.] -- Identification of HIV-1 reverse transcriptase inhibitors using a scintillation proximity assay / Bertrand Van Schoubroeck ... [et al.] -- Biochemical screening assays to identify HIV-1 integrase inhibitors / Marleen Clynhens ... [et al.] -- HIV-1 genotyping of the protease-reverse transcriptase and integrase genes to detect mutations that confer antiretroviral resistance / Peter Van den Eede ... [et al.] -- Part II Hepatitis viruses -- In vitro kinetic profiling of hepatitis C virus NS3 protease inhibitors by progress curve analysis / Rumin Zhang and William T. Windsor -- Novel hepatitis C virus NS5B polymerase assay of de novo initiated RNA synthesis directed from a heteropolymeric RNA template / Eric Ferrari and Hsueh-Cheng Huang -- Selecting and characterizing drug-resistant hepatitis C virus replicon / Inge Vliegen, Leen Delang, and Johan Neyts -- Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors / Leen Vijgen ... [et al.] -- Expression and purification of hepatitis C virus protease from clinical samples / Liesbet van der Helm -- Surface plasmon resonance as a tool to select potent drug candidates for hepatitis C virus NS5B polymerase / Erna Cleiren, Benoit Devogelaere, and Katleen Fierens -- Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b / Diana Koletzki ... [et al.] -- Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures / Dawei Cai ... [et al.] -- In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates / Yang Liu and Kathryn M. Kitrinos -- Part III Flaviviruses -- Cell-based antiviral assays for screening and profiling inhibitors against dengue virus / Edwin Yunhao Gong ... [et al.] -- Duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes / Edwin Yunhao Gong ... [et al.] -- High-throughput screening using dengue virus reporter genomes / Wolfgang Fischl and Ralf Bartenschlager -- Fluorimetric and HPLC-based dengue virus protease assays using a FRET substrate / Christoph Nitsche and Christian D. Klein -- Expression and purification of dengue virus NS5 polymerase and development of a high-throughput enzymatic assay for screening inhibitors of dengue polymerase / Edwin Yunhao Gong ... [et al.] -- Detection and quantification of flavivirus NS5 methyl- transferase activities / Siew Pheng Lim, Christophe Bodenreider, and Pei-Yong Shi -- Testing antiviral compounds in a dengue mouse model / Wouter Schul, Andy Yip, and Pei-Yong Shi -- Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds / Sofia L. Alcaraz-Estrada, Erin Donohue Reichert, and Radhakrishnan Padmanabhan -- Part IV Herpes viruses -- Methods for screening and profiling inhibitors of herpes simplex viruses / Edwin Yunhao Gong -- In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models / Frances Valencia, Ronald L. Veselenak, and Nigel Bourne -- Fluorescence-based high-throughput screening assay for identifying human cytomegalovirus inhibitors / Christel Van den Eynde ... [et al.] -- Part V Respiratory viruses -- Fluorescence-based high-throughput antiviral compound screening assay against respiratory syncytial virus / Leen Kwanten, Ben De Clerck, and Dirk Roymans -- Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells / Anna Lundin, Tomas Bergström, and Edward Trybala -- Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models / Joke Van den Berg, Leen Kwanten, and Dirk Roymans -- In vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (Chlorocebus pygerythrus) infection model / Lieselot Houspie ... [et al.] -- Methods to determine mechanism of action of anti-influenza inhibitors / Angela Luttick, Stephanie Hamilton, and Simon P. Tucker -- Methods for evaluation of antiviral efficacy against influenza virus infections in animal models / Donald F. Smee and Dale L. Barnard -- Part VI Alphaviruses -- Development of a high-throughput antiviral assay for screening inhibitors of chikungunya virus and generation of drug-resistant mutations in cultured cell / Edwin Yunhao Gong ... [et al.] -- Mouse model of chikungunya virus with utility in antiviral studies / Ashley Dagley and Justin G. Julander. edited by Edwin Yunhao Gong. Includes bibliographical references and index. Antiviral Agents RM411 .A5755 2013 615.7924 ANM 9781627034838 (alk. paper) 1627034838 (alk. paper) 2013940287 BTCTA 130514 20150408114225.0 17737724 eng