RNA therapeutics : function, design, and delivery / edited by Mouldy Sioud. - New York : Humana Press, c2010. - xvi, 526 p. : ill. ; 27 cm. - Methods in molecular biology, 629 1064-3745 ; Springer protocols . - Methods in molecular biology (Clifton, N.J.) ; v. 629. Springer protocols. .

Includes bibliographical references and index.

RNA modifications : a mechanism that modulates gene expression / John Karijolich, Athena Kantartzis, and Yi-Tao Yu -- Quantitative analysis of RNA modifications / John Karijolich, Athena Kantartzis, and Yi-Tao Yu -- Advances in RNA sensing by the immune system : separation of siRNA unwanted effects from RNA interference / Mouldy Sioud -- Progress in siRNA delivery using multifunctional nanoparticles / Weiwei Gao ... [et al.] -- Optimized protocols for siRNA delivery into monocytes and dendritic cells / Anne Mobergslien and Mouldy Sioud -- Systemic delivery of synthetic siRNAs / Dag R. Sørensen and Mouldy Sioud -- What are the key targeted delivery technologies of siRNA now? / Mouldy Sioud -- Using OligoWalk to identify efficient siRNA sequences / David H. Mathews -- Jet-injections of short hairpin RNA-encoding vectors into tumor cells / Wolfgang Walther, Ulrike Stein, and Hermann Lage -- Short hairpin RNA (shRNA) : design, delivery, and assessment of gene knockdown / Chris B. Moore ... [et al.] -- Effective Pol III-expressed long hairpin RNAs targeted to multiple unique sites of HIV-1 / Sheena M. Saayman, Patrick Arbuthnot, and Marc S. Weinberg -- Functional studies on RNA-transfected cell microarrays / Christina Sæten Fjeldbo ... [et al.] -- Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration / Reiko Onuki-Nagasaki ... [et al.] -- Intron-mediated RNA interference, intronic microRNAs, and applications / Shao-Yao Ying, Chen Pu Chang, and Shi-Lung Lin -- Inhibition of the microRNA pathway in zebrafish by siRNA / Anders Fjose and Xiao-Feng Zhao -- Profiling of miRNA expression and prediction of target genes / Mouldy Sioud and Lina Cekaite -- Small RNA cloning / Seungil Ro and Wei Yan -- In situ hybridization detection of microRNAs / Rui Song, Seungil Ro, and Wei Yan -- Detection and quantitative analysis of small RNAs by PCR / Suengil Ro and Wei Yan -- In vivo reprogramming of human telomerase reverse transcriptase (hTERT) by trans-splicing ribozyme to target tumor cells / Sang-Jin Lee ... [et al.] -- Design and function of triplex hairpin ribozymes / Guillermo Aquino-Jarquin, Ramiro Rojas-Hernández, and Luis Marat Alvarez-Salas -- Catalytic M1GS RNA as an antiviral agent in animals / Yong Bai, Paul Jay Fannin Rider, and Fenyong Liu -- Using live cells to generate aptamers for cancer study / Ling Meng ... [et al.] -- Primer-free aptamer selection using a random DNA library / Weihua Pan and Gary A. Clawson -- Development of TLR7/8 small RNA antagonists / Mouldy Sioud -- Modulation of dendritic cell maturation and function by siRNA-bearing 5'-triphosphate / Mouldy Sioud -- Immunotherapy of cancer with dendritic cells loaded with tumor antigens and activated through mRNA electroporation / An M.T. Van Nuffel ... [et al.] -- Non-MHC-dependent redirected T cells against tumor cells / Hilde Almåsbak, Marianne Lundby, and Anne-Marie Rasmussen -- Overcoming self-tolerance to tumour cells / Mouldy Sioud -- Recent advances in hematopoietic stem cell transplantation and perspectives of RNAi applications / Yngvar Fløisand and Mouldy Sioud.

9781607616566 (hbk. : alk. paper) 1607616564 (hbk. : alk. paper) 9781607616573 (eISBN) 1607616572 (eISBN)

2010921201

GBB067997 bnb

101550754 DNLM 015567081 Uk


RNA--Therapeutic use.
Small interfering RNA--Therapeutic use.
RNA--Therapeutic use--Laboratory manuals.
RNA Interference.
RNA, Small Interfering--therapeutic use.
Gene Therapy.
Neoplasms--therapy.

RM666.R554 / R53 2010

615.31 / RNT

RM666.R554 / R63 2010

W1 / ME9616J v.629 2010 QU 58.7